Exploring the regulation of gene expression in eukaryotic cells

The topic involves the development and use of novel techniques of high throughput gene expression (microarrays, GFP expression and targeting, flow cytometry and cell sorting) 

 

主讲人:

David W. Galbraith

Professor of Plant Sciences
Professor of Bio5 Institute
University of Arizona
Email: galbraith@arizona.edu
http://cals.arizona.edu/galbraith

主讲人简历:

David W. Galbraith

Education:

Cambridge University, England. BA 1970-73 Biochemistry
Cambridge University, England. MA, PhD 1973-76 Biochemistry

Professional Experience:

1976-78:  NATO Science Research Fellowship, Department of Biological Sciences, Stanford University.
1978-82:  Assistant Professor, Section of Genetics, Molecular and Cellular Biology, School of Biological Sciences, University of Nebraska-Lincoln. 
1982-1988:  Associate Professor, Sections of Genetics, Molecular and Cellular Biology and Molecular Plant Biology, School of Biological Sciences, University of Nebraska-Lincoln. Courtesy appointment, Dept. of Agronomy.
1989-present:  Professor, Department of Plant Sciences, University of Arizona. 
1998-present:  Member, University of Arizona Cancer Center. 
2004-present:  Professor, Bio5 Institute, University of Arizona.

Selected publications:

Birnbaum, K., Shasha, D.E., Wang, J.Y., Jung, J.W., Lambert, G.M., Galbraith, D.W., and Benfey, P.N. (2003). A gene expression map of the Arabidopsis root. Science 302:1956-1960

Gardiner, J.M, Buell, C.R., Elumalai, R., Galbraith, D.W., Henderson, D.A., Iniguez, A.L., Kaeppler, S.M., Kim J.J., Liu, J., Zheng, L., and Chandler, V.L. (2005). Design, production, and utilization of long oligonucleotide microarrays for expression analysis in maize. Maydica 50:425-435.

Ammiraju, J.S.S., Luo, M., Wang, W., Kudrna, D., Goicoechea, J.L., Mueller, C., Talag, J., Kim, H.R., Sisneros, N.B., Blackmon, B., Fang, E., Tomkins, J., Brar, D., MacKill, D., McCouch, S., Kurata, N., Lambert, G., Galbraith, D.W., Arumuganathan, K., Rao, K., Walling, J.G., Gill, N., SanMiguel, P., Soderlund, C., Jackson, S., and Wing, R.A. (2006). The Oryza Map Alignment Project (OMAP) bacterial artificial chromosome library resource: unveiling a new era in whole genome comparative genomics to study monocot plant evolution, development, and physiology. Genome Research 16:140-147.

Song, C-P., and Galbraith, D.W. (2006). AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis. Plant Molecular Biology 60:241–257.

Galbraith, D.W., and Birnbaum, K. (2006). Global studies of cell type-specific gene expression in plants. Annual Reviews of Plant Biology 57:451-475.

Rangwala, S.H., Elumalai, R., Vanier, C., Ozkan, H., Galbraith, D.W., and Richards, E.J. (2006). Meiotically-stable natural epigenetic variation in Sadhu, a novel Arabidopsis retroposon. PLOS Genetics 2:270-281.

Barthelson, R.A., Sundareshan, P., Galbraith, D.W., and Woosley, R.L. (2006). Development of a comprehensive detection method for medicinal and toxic plant species. American Journal of Botany 93:566–574.

Ozkan, H., Tuna, M., and Galbraith, D.W. (2006). No DNA loss in autotetraploids of Arabidopsis thaliana. Plant Breeding 125:288-291.

Li, J., Li, X., Su, H., Chen, H., and Galbraith, D.W. (2006). A framework of integrating gene relations from heterogeneous data sources: an experiment on Arabidopsis thaliana. Bioinformatics 22:2037-2043.

Galbraith, D.W. (2006). The daunting prospect of MIAME. Nature 444:31.

Galbraith, D.W. (2006). Microarray analyses in higher plants. OMICS: A Journal of Integrative Biology 10:455-473.

Galbraith, D.W. (2007). Protoplast analysis using flow cytometry and sorting. In: Dolezel, J., Greilhuber, J., Suda, J. (eds.): Flow Cytometry with Plant Cells, Wiley-VCH, pp. 231-250.

Galbraith, D.W. (2007). Analysis of plant gene expression using flow cytometry and sorting. In: Dolezel, J., Greilhuber, J., Suda, J. (eds.): Flow Cytometry with Plant Cells, Wiley-VCH, pp.405-422. Agrawal, A., Cronin, J.P., Tonazzi, J.C., Goodyear, A. G., LeCompte, R.S., Hogan, M.E.,

Galbraith, D.W. (2007). High surface area substrates for microarrays and methods to make same. U. S. Dept. of Commerce Patent 7,195,872.

Galbraith, D.W. (2007). Silica breaks through in plants. Nature Nanotechology 2:272-273.

 

时间: 2007年5月24日(星期)上午 9:30-11:00

 

地点:中国农业大学西校区图书馆报告厅

 

联系人:苏震(62731380

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